Features of the carbons in deoxyribose: `1'`, `2'`, `3'`, `5'` 1': nitrogenous base 2': no oxygen 3': hydroxyl group 5': phosphoester Type of bond in backbone phosphodiester In DNA phosphoester gets what charge (loses hydrogen in OH) negative The longer base type is `type`, with bases `baselong`, the shorter is `shorttype`, with bases `baseshort` type: purine baselong: AG shorttype: pyrimidine baseshort: CT Which pair of bases have double hydrogen bonds instead of triple hydrogen bonds A T Type of reaction to make DNA polymers condensation What type of model do we have for DNA replication (experiment shows that resulting DNA is mostly non radioactive or whatever) semiconservative How many origins of replication do bacteria have 1 Steps of DNA: `1`, `2`, `3` 1: initiation 2: elongation 3: termination In initiation what enzyme splits the DNA helicase What stops the DNA from re-binding single-strand binding proteins What stops the DNA thats getting twisted up in front and behind of the replication bubble from breaking topoisomerases Notable topoisomerase gyrase What adds the RNA primer needed for elongation primase In elongation, what enzyme elongates from the RNA primer DNA Polymerase III After the DNA Pol III elongates what replaces the RNA primer with DNA (termination) DNA Polymerase I What direction does DNA form 5' to 3' What is direction of the leading strand going towards the replication fork 3' to 5' What enzyme connects the end of the elongated DNA segment to the DNA primer (termination) ligase Who showed that transformation occurs with DNA by combining heated (dead) virulent bacteria with nonvirulent living cells and having those nonvirulent ones kill mice (therefore showing they reproduced with the virulent DNA) Griffith Chargaff's 2 rules: `varies` varies and `same` is the same varies: A/G ratio same: C/G ratio When bases are matched incorrectly and don't stick it is called `name`, caused by `cause` name: thymine dimer cause: UV radiation